Polypeptides having colanic acid-degrading activity
AssigneeGradalis, Inc.
InventorNancy Smyth Templeton
Life of this patent
Estimating the term…
Abstract
The present disclosure generally relates to polypeptides having colanic acid-degrading activity and methods of using the same. Polynucleotides encoding such polypeptides are also described. The polypeptides may be used, for example, in processes for degrading colanic acid, processes for the removal of endotoxins from biological samples, and processes for purifying plasmid DNA.
Claims24
An isolated polynucleotide comprising a nucleic acid sequence that encodes a polypeptide having colanic acid degrading activity having at least 98% sequence identity with SEQ ID NO:
- 1, or the complement thereof.
An isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide having colanic acid degrading activity having at least 98% sequence identity with SEQ ID NO:
- 2, or the complement thereof.
A method of making a recombinant polypeptide comprising:
- transforming a cell with a recombinant vector comprising a nucleic acid sequence encoding a polypeptide having colanic acid degrading activity and at least 98% sequence identity with at least one of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, and growing the cell under conditions in which the cell expresses the polypeptide.
A host cell transformed with a recombinant vector that comprises a polynucleotide comprising a nucleic acid sequence encoding a polypeptide having colanic acid degrading activity and at least 98% sequence identity with at least one of SEQ ID NO:1 or SEQ ID NO:2, wherein the nucleic acid is operably linked to a heterologous promoter.
Dependencies are inferred from the claim text.