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AssigneeToyota Jidosha Kabushiki Kaisha
InventorsMasayoshi Muramatsu, Kazuyo Suzuki
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This invention is intended to produce 2-butanol with excellent productivity via a fermentation process. Recombinant microorganisms into which the acetoacetyl-CoA synthase gene and a group of genes (i.e., genes involved in 2-propanol synthesis) encoding a set of enzymes synthesizing 2-propanol from acetoacetyl-CoA have been introduced are cultured, so that, in addition to 2-propanol, 2-butanol is produced at a high level in a medium.
A method for producing 2-butanol comprising culturing in a culture medium containing a carbon source, a recombinant microorganism into which an acetoacetyl-CoA synthase gene, the acetoacetyl-CoA transferase gene, the acetoacetic acid decarboxylase gene, and the isopropanol dehydrogenase gene have been introduced and obtaining 2-butanol from the culture product.
Dependencies are inferred from the claim text.
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Acyclic: butanols
Oxidoreductases: acting on CH-OH groups as donors
Acyltransferases: transferring groups other than amino-acyl groups
Transferases: transferring sulfur containing groups
About 4 years 10 months of term left · ends 15 Aug 2031 (estimated)
1. Field of the Invention
The present invention relates to a method for producing butanol using a recombinant microorganism having butanol production capacity, into which genes involved in 2-butanol (or sec-butyl alcohol) biosynthesis have been incorporated, and a recombinant microorganism.
2. Background Art
FIG. 1 is a characteristic diagram showing the results of GC-MS analysis of the culture solution of a recombinant E. coli strain.
FIG. 2 is a characteristic diagram showing the results of GC-MS analysis of standard substances.
FIG. 3 is a characteristic diagram showing the results of quantification of the amounts of 2-butanol and other substances produced by the recombinant E. coli E/BL21 strain.
Hereafter, the present invention is described in detail.
The method for producing 2-butanol (or sec-butyl alcohol) of the present invention comprises culturing a recombinant microorganism into which the acetoacetyl-CoA synthase gene and genes involved in 2-propanol biosynthesis associated with a metabolic pathway for synthesizing 2-propanol from acetoacetyl-CoA have been introduced and obtaining 2-butanol from the culture product.
Acetoacetyl-CoA Synthase Gene