Enzymatic antibody processing
AssigneeHoffman-La Roche Inc.
InventorsMarkus Haberger, Christine Jung, Dietmar Reusch
Life of this patent
Estimating the term…
Abstract
The current invention comprises a method for producing an immunoglobulin or immunoglobulin fragment with defined glycostructure comprising the following steps: a) providing an affinity chromatography column eluate containing the immunoglobulin or immunoglobulin fragment, b) incubating the affinity chromatography column eluate with (α1,3)galactosidase of plant origin, e.g. from green coffee beans (EC 3.2.1.22), c) applying the incubated affinity chromatography column eluate to a protein A chromatography material and recovering the immunoglobulin or immunoglobulin fragment from the protein A chromatography material and thereby producing an immunoglobulin or immunoglobulin fragment with defined glycostructure.
Claims10
A method for producing an immunoglobulin or polypeptide comprising at least the CH2 domain and the CH3 domain of a full length immunoglobulin heavy chain with defined glycostructure comprising the following steps:
- providing an affinity chromatography column eluate containing the immunoglobulin or polypeptide;
- and
- incubating the affinity chromatography column eluate with an α-galactosidase of plant origin, which cleaves off the (α1,3) glycosidically bound galactose residue at the non-reducing end of the glycostructure in the CH2 domain of the immunoglobulin or polypeptide.
Dependencies are inferred from the claim text.