Two-step gram staining method
AssigneeYing Ye
InventorsYing Ye, Tao Yuan, Li-Xin Yang, Feng-Qi Wen
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Abstract
The present invention provides a two-step Gram staining method. The method is conducted by using a clean slide, followed by adding 10˜30 μl primary stain from a reagent kit, adding an equivalent amount of specimens uniformly on said slide, then immediately adding 30˜60 μl counter-stain on said slide for incubating 15 seconds, rinsing with water, and finally examining under a microscope, the result appears color contrast on the center of the slide between the Gram-negative bacteria performing light red color and the Gram-positive bacteria performing deep purple color.
Claims5
A Gram staining method, consisting of a primary staining step and a counter-staining step, and the primary staining step and the counter-staining step further comprising the sub-steps of:
- adding 10˜30 μl primary stain obtained from a reagent kit on a clean slide;
- adding specimens, volume of which is equivalent to said primary stain and coating uniformly on said slide, wherein said specimens include Gram-negative bacteria and Gram-positive bacteria;
- adding 30˜60 μl counter-stain on said slide for incubating 10˜20 seconds immediately;
- rinsing said slide with water and then drying said slide;
- and
- examining said slide under a microscope, wherein results appear that color contrast is present on the center of the slide in between said Gram-negative bacteria performing light red color and said Gram-positive bacteria performing deep purple color;
- wherein said primary stain consists of 0.2 to 0.5 wt. % crystal violet, and 4 to 6 wt. % sodium chloride solvent, and said counter-stain consists of 0.2 to 0.5 wt. % basic fuchsin, 0.1 to 0.3 wt. % potassium iodide, 0.05 to 0.15 wt. % iodine and 90 to 96 wt. % ethyl alcohol.
Dependencies are inferred from the claim text.