Microvesicle membrane protein and application thereof
OwnerMing-Chung Jianginventor
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Abstract
Microvesicles play essential roles in disease progression. The present invention provides a microvesicle membrane protein and application thereof. Disclosed is method comprises phosphorylated CSE1L (cellular apoptosis susceptibility protein- or CSE1L-binding agents for microvesicle isolation, analysis, or binding for disease diagnosis or treatment.
Claims9
An in vitro method of measuring the levels of chromosome segregation 1-like protein (CSE1L) or phosphorylated CSE1L in microvesicles in biological fluid of a test subject comprising the steps of:
- (a) obtaining a test biological fluid sample from a test subject;
- (b) isolating microvesicles in the test biological fluid sample;
- (c) contacting the microvesicles with an antibody against CSE1L or an antibody that specifically recognizes phosphorylated CSE1L and does not recognize non-phosphorylated CSE1L;
- and
- (d) determining the amount of CSE1L or phosphorylated CSE1L in microvesicles in the test biological fluid sample.
An in vitro method of measuring the level of phosphorylated CSE1L in body fluids from a test subject comprising the steps of:
- (a) obtaining a test biological fluid sample from a test subject;
- (b) contacting the test biological fluid sample with an antibody that specifically recognizes phosphorylated CSE1L and does not recognize non-phosphorylated CSE1L;
- and
- (c) determining the amount of phosphorylated CSE1L in the test biological fluid sample.
Dependencies are inferred from the claim text.