Multiple antigenic peptide assay for detection of HIV or SIV type retroviruses

An enzyme immunoassay construct for detecting a primate immunodeficiency virus, comprising:

• a plurality of detection multiple antigenic peptides comprising a portion of an immunodominant region (IDR) of a transmembrane protein of a primate immunodeficiency virus and the plurality of detection multiple antigenic peptides comprising a detection multiple antigenic peptide comprising an SIVcpz IDR, a detection multiple antigenic peptide comprising an SIVsyk IDR and a detection multiple antigenic peptide comprising an SIVdeb IDR;
• and
• a plurality of differentiation multiple antigenic peptides comprising a portion of a V3-loop of an envelope protein of a primate immunodeficiency virus and the plurality of differentiation multiple antigenic peptides comprise a differentiation multiple antigenic peptide comprising a portion of a simian immunodeficiency virus (SIV)cpz V3-loop, a differentiation, multiple antigenic peptide comprising a portion of a SIVsyk V3-loop and a differentiation multiple antigenic peptide comprising a portion of a SIVdeb V3-loop,
• wherein the plurality of detection multiple antigenic peptides and the plurality of differentiation multiple antigenic peptides are immobilized on a single substrate respectively;
• wherein each of the detection multiple antigenic peptide consists of a core matrix and at least two linear antigenic sequences bonded to the core matrix by β-alanine and d-aspartic acid, each linear antigenic sequence is less than 16 amino acid residues and the β-alanine and d-aspartic acid serving as spacer amino acids between each linear antigenic sequence and the core matrix;
• wherein each of the differentiation multiple antigenic peptide consists of a core matrix and at least two linear antigenic sequences bonded to the core matrix by diaminopropionic acid, each linear antigenic sequence is less than 16 amino acid residues and diminopropionic acid serving as a spacer amino acid between each linear antigenic sequence and the core matrix.

An enzyme immunoassay construct for detecting a primate immunodeficiency virus, comprising:

• a first substrate to which is bound a plurality of detection multiple antigenic peptides, each detection multiple antigenic peptide comprising a portion of an immunodominant region of a transmembrane envelope protein of a primate immunodeficiency virus, wherein at least one simian immunodeficiency virus is represented in at least one of the detection multiple antigenic peptides;
• and
• a second substrate to which is bound a plurality of differentiation multiple antigenic peptides, each differentiation multiple antigenic peptide comprising a portion of a V3-loop of an envelope protein of a primate immunodeficiency virus, wherein at least one simian immunodeficiency virus is represented in at least one of the differentiation multiple antigenic peptides;
• wherein the detection multiple antigenic peptides comprise of a core matrix and four linear detection antigenic sequences bonded to the core matrix by β-alanine and d-aspartic acid, each linear detection antigenic sequence is WGCSGKAVCYT (SEQ ID NO:
• 1) and the β-alanine and d-aspartic acid serve as spacer amino acids between each linear detection antigenic sequence and the core matrix;
• and
• wherein the differentiation multiple antigenic peptides comprise of a core matrix and four linear antigenic sequences bonded to the core matrix by diaminopropionic acid, each differentiation linear antigenic sequence is RGEVQIGPGMTFYNI (SEQ ID NO:
• 14) and diminopropionic acid serves as a spacer amino acid between each differentiation linear antigenic sequence and the core matrix.