Method for the in vitro diagnosis of stroke

A method for the in vitro diagnosis of stroke and transient ischemic attack (TIA) in an individual, comprising the following steps:

• (a) measuring the level of proBNP(1-108), or of fragments of proBNP(1-108) comprising a RAPRSP sequence (SEQ ID NO:
• 1), in a biological sample of the individual;
• (b) comparing the measured level with a cut-off value;
• (c) determining therefrom whether a stroke or a TIA has occurred in the individual,
• wherein a measured level higher than the cut-off value is indicative of a stroke or a TIA;
• and
• wherein the level of proBNP(1-108), or of fragments of proBNP(1-108), i determined using an immunoassay which comprises an antibody targeting an epitope which comprises the RAPRSP sequence (SEQ ID NO:
• 1),
• wherein the antibody targeting an epitope which comprises the RAPRSP sequence (SEQ ID NO:
• 1) is secreted by the hybridoma deposited at the CNCM (Paris, France) under the Budapest Treaty on Apr. 29, 2005, under reference number I-3073.

A method for treating at least one of stroke and transient ischemic attack (TIA) in an individual having a level of proBNP(1-108) or of fragments of proBNP(1-108) comprising a RAPRSP sequence (SEQ ID NO:

• 1) in a biological sample higher than a cut-off value, comprising the following steps:
• (a) measuring the level of proBNP(1-108), or of fragments of proBNP(1-108) comprising a RAPRSP sequence (SEQ ID NO:
• 1), in a biological sample of the individual;
• (b) comparing the measured level with a cut-off value;
• and
• (c) if the measured level is higher than the cut-off value, administrating to said individual a therapeutically efficient amount of clot-dissolving agents.