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US 8,524,458 B2Granted
Secretory protein biomarkers for high efficiency protein expression
AssigneeAbbVie Inc.
InventorsPatrick Hossler, Ivan Correia
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Abstract
The instant invention relates to the field of protein production, and in particular is relates to compositions and processes for improving the production levels of recombinant proteins expressed in host cells.
Claims16
A method of identifying a high efficiency cell or cell culture comprising:
- (a) determining the expression level of a high efficiency cell or cell culture biomarker panel in the cell or cell culture, wherein said biomarker panel comprises guanine binding protein subunit beta 1, heat shock protein beta 1, and 14-3-3 protein zeta/delta, and
- (b) comparing the expression level of that high efficiency cell or cell culture biomarker panel in the cell or cell culture to the expression level of the biomarker panel in a known high efficiency cell or cell culture,
- wherein an expression level of heat shock protein beta 1, and 14-3-3 protein zeta/delta higher than the level in the known high efficiency cell or cell culture and an expression level of guanine nucleotide binding protein subunit beta 1 less than the level in the known high efficiency cell or cell culture is indicative of a high efficiency cell or cell culture.
A kit for identifying modulation in expression of a secretory protein biomarker of high efficiency protein expression comprising:
- (a) multiple detectable antibodies and wherein each antibody specifically binds to a secretory protein of a biomarker panel of high efficiency protein expression, wherein the biomarker panel comprises guanine nucleotide binding protein subunit beta 1, heat shock protein beta 1, and 14-3-3 protein zeta/delta;
- and
- (b) a means for detecting said antibody.
Dependencies are inferred from the claim text.