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US 8,524,453 B2Granted
Lectin complement pathway assays and related compositions and methods
AssigneeThe Brigham and Woman's Hospital, Inc.
InventorsGregory L. Stahl, Mary C. Walsh
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Abstract
This invention is related, in part, to assays for analyzing the lectin complement pathway (LCP) as well as to compositions and methods related thereto.
Claims24
A method of assessing lectin complement pathway (LCP) activation, comprising:
- determining an amount of mannose binding lectin (MBL) in a biological sample,
- removing non-covalently bound MBL/mannose binding lectin serine protease 2 (MASP-2) complexes,
- determining an amount of another LCP component in the biological sample, and
- assessing LCP activation in the biological sample based on the amounts of MBL and the another component.
1 more element
A method of assessing LCP activation, comprising:
- contacting a substrate coated with a ligand of MBL with a biological sample,
- contacting the sample with a detectably labeled agent that specifically binds MBL,
- determining the amount of MBL present,
- removing non-covalently bound MBL/MASP-2 complexes from the sample,
- contacting the sample with a detectably labeled agent that specifically binds C4,
- contacting the sample with a detectably labeled agent that specifically binds C3,
- determining the amount of C4 and C3 present, and
- assessing LCP activation in the sample based on the amounts of MBL, C4 and C3.
5 more elements
Dependencies are inferred from the claim text.