Selective lysing of cells using ultrasound
AssigneeSound Surgical Technologies LLC
InventorMark E. Schafer
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Abstract
Described are embodiments that employ ultrasonic energy to selectively lyse larger adipose cells in a suspension containing adipose cells of different sizes resulting in a suspension in which the only viable cells are the small adipose cells and stem cells. Embodiments provide for generating an acoustic standing wave field of sufficient intensity and proper geometry, that high shear stress is induced on the cell membranes of cells larger than a predetermined size. The remaining small adipose cells can be physically separated from the suspension after the suspension is subjected to the acoustic standing wave field.
Claims16
A method for lysing adipose cells of diameters equal to or greater than a predetermined diameter, the method comprising:
- generating an acoustic standing wave field, wherein the geometry of the acoustic standing wave field is such that one half of the wavelength of the acoustic standing wave field is less than or equal to the predetermined diameter, and wherein the intensity of the acoustic standing wave field is sufficient to cause lysing of adipose cells of diameters equal to or greater than the predetermined diameter;
- and
- subjecting a mixture of adipose cells and stem cells to the acoustic standing wave field to generate a lysed mixture, wherein the mixture of adipose cells and stem cells includes at least some adipose cells having a diameter equal to or greater than the predetermined diameter.
A method for lysing adipose cells of diameters equal to or greater than a predetermined diameter, the method comprising:
- generating an acoustic standing wave field, wherein the geometry of the acoustic standing wave field is such that one half of the wavelength of the acoustic standing wave field is less than or equal to the predetermined diameter, the wavelength of the acoustic standing wave field falling within a range of 0.5 mm to 2.5 mm, and wherein the intensity of the acoustic standing wave field is sufficient to cause lysing of adipose cells of diameters equal to or greater than the predetermined diameter;
- and
- subjecting a mixture of adipose cells and stem cells to the acoustic standing wave field to generate a lysed mixture, wherein the mixture of adipose cells and stem cells includes at least some adipose cells having a diameter equal to or greater than the predetermined diameter.
Dependencies are inferred from the claim text.