Production of proteins in glutamine-free cell culture media
OwnerMartin Gawlitzek, Shun Luo +1inventors
InventorsMartin Gawlitzek, Shun Luo, Christina Teresa Petraglia
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Abstract
The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.
Claims25
A process for producing a polypeptide in a mammalian host cell expressing said polypeptide, comprising culturing the mammalian host cell in a production phase of the culture in a glutamine-free production culture medium containing asparagine, wherein the asparagine is added at a concentration in the range of 7.5 mM to 15 mM.
A ready-to-use glutamine-free cell culture medium containing asparagine for the production of a polypeptide in a production phase, wherein the asparagine is at a concentration in the range of 7.5 mM to 15 mM.
Dependencies are inferred from the claim text.