Optimized method for LID biosensor resonance detection
AssigneeCorning Incorporated
InventorsJacques Gollier, Garrett A. Piech, Michael B. Webb, Qi Wu
Life of this patent
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Abstract
An optical interrogation system is described herein that can interrogate a label-independent-detection (LID) biosensor and monitor a biological event on top of the biosensor without suffering from problematical parasitic reflections and/or problematical pixelation effects. In one embodiment, the optical interrogation system is capable of interrogating a biosensor and using an oversampling/smoothing algorithm to reduce oscillations in the estimated location of an optical resonance caused by the problematical pixelation effect which makes it easier to determine whether or not a biological event occurred on the biosensor.
Claims28
An optical interrogation system for interrogating a label-independent biosensor, the optical interrogation system comprising:
- a launch system that is arranged to emit an optical beam towards a biosensor;
- a receive system that is arranged to collect an optical beam from the biosensor and then to output a signal which is representative of the collected optical beam;
- a processor that is arranged to reduce pixelation oscillations in an optical resonance of the signal by oversampling at least a portion of the optical resonance, smoothing the optical resonance and calculating a resonance location or centroid that is based on the smoothed-oversampled optical resonance;
- and
- the processor is further arranged to monitor a biological event or a biochemical event on a top surface of the biosensor by sensing a shift between one calculated resonance location or centroid based on one smoothed-oversampled optical resonance and another calculated resonance location or centroid based on another smoothed-oversampled optical resonance.
A method for interrogating a label-independent biosensor, said method comprising the steps of:
- emitting an optical beam towards a biosensor;
- collecting an optical beam from the biosensor
- generating a signal which corresponds to the collected optical beam;
- and
- reducing oscillations in an optical resonance of the signal caused by a pixelation effect by:
- oversampling at least a portion of the optical resonance;
- smoothing the optical resonance;
- and
- calculating a resonance location or centroid that is based on the smoothed-oversampled optical resonance;
- and
- monitoring a biological event or a biochemical event on a top surface of the biosensor by sensing a shift between one calculated resonance location or centroid based on one smoothed-oversampled optical resonance and another calculated resonance location or centroid based on another smoothed-oversampled optical resonance.
An optical interrogation system comprising:
- a launch system that emits an optical beam towards a resonance waveguide grating biosensor;
- a receive system that collects an optical beam from the resonance waveguide grating biosensor and then outputs a signal which corresponds to the collected optical beam;
- and
- a processor that applies a low pass filter to the signal, where the low pass filter has a selected low pass filter width which is wide enough to digitally remove parasitic fringes generated by presence of parasitic reflections located on each side of an optical resonance represented within the signal by applying the low pass filter to the signal, where the parasitic reflections are caused by the optical beam which passes through a substrate of the resonance waveguide grating biosensor and is then reflected from a top surface of the resonance waveguide grating biosensor.
A method for interrogating a resonance waveguide grating biosensor, said method comprising the steps of:
- emitting an optical beam towards the resonance waveguide grating biosensor;
- collecting an optical beam from the resonance waveguide grating biosensor;
- generating a signal which corresponds to the collected optical beam;
- and
- applying a low pass filter to the signal, where the low pass filter has a selected low pass filter width which is wide enough to digitally remove parasitic fringes generated by presence of parasitic reflections located on each side of an optical resonance represented within the signal by applying the low pass filter to the signal, where the parasitic reflections are caused by the optical beam which passes through a substrate of the resonance waveguide grating biosensor and is then reflected from a top surface of the resonance waveguide grating biosensor.
Dependencies are inferred from the claim text.