Method for measuring a concentration of a biogenic substance contained in a living body

A method for measuring a concentration of a biogenic substance contained in a living body, the method comprising steps of:

• a step (a) of preparing a measuring device comprising a light source, a focusing controller, an optical filter, and a light receiver;
• a step (b) of transmitting a first focused light from the light source through the focusing controller, and focusing the first focused light on a surface of a particle chip implanted in a skin though a first area on the surface of the skin so as to generate a first reflected light, wherein
• the particle chip comprises a substrate and a plurality of metal particles,
• a step (c) of receiving the first reflected light by the light receiver through the optical filter to obtain a first signal Xa,
• the following equation (III) is satisfied:
• lambda2=(107*lambda1)/(107−B*lambda1) (III),
• lambda2:
• the central wavelength of optical filter,
• lambda1:
• the wavelength of the first focused light,
• B:
• Raman shift of the biogenic substance;
• a step (d) of transmitting a second focused light from the light source through the focusing controller, and irradiating the surface of the particle chip with the second focused light through a second area on the surface of the skin so as to generate a second reflected light, wherein
• the focal point of the second focused light is different from the focal point of the first focused light,
• the first area is identical to the second area, and
• a step (e) of receiving the second reflected light by the light receiver through the optical filter to obtain a second signal Xb,
• a step (f) of calculating the concentration of the biogenic substance on the basis of the difference between the first signal Xa and the second signal Xb.