Methods for altering the reactivity of plant cell walls
AssigneeBayer CropScience AG
InventorsMarc De Block, Frank Meulewaeter, Rainhard Koch, Bernd Essigmann
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Abstract
Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.
Claims16
A chimeric gene, comprising the following operably linked DNA regions:
- a.) a plant-expressible promoter;
- b.) a DNA region coding for an N-acetylglucosamine transferase, wherein said N-acetylglucosamine transferase is a chitin synthase comprising a signal anchor sequence for targeting the membranes of the Golgi apparatus or wherein said N-acetylglucosamine transferase is of the NODC type, and wherein said N-acetylglucosamine transferase comprises the amino acid sequence of SEQ ID No. 15 from position 1 to position 35;
- and
- c.) a transcription termination and polyadenylation region.
A chimeric gene, comprising the following operably linked DNA regions:
- a.) a plant-expressible promoter;
- b.) a DNA region coding for an N-acetylglucosamine transferase, wherein said N-acetylglucosamine transferase is a chitin synthase comprising a signal anchor sequence for targeting the membranes of the Golgi apparatus or wherein said N-acetylglucosamine transferase is of the NODC type, and wherein said N-acetylglucosamine transferase comprises the amino acid sequence of SEQ ID No. 15;
- and
- c.) a transcription termination and polyadenylation region.
Dependencies are inferred from the claim text.