Back to results
US 8,507,748 B2Granted
Method for the production of a single heavy chain antibody
AssigneeErasmus Universiteit Rotterdam
InventorFrank Grosveld
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for the generation of single chain immunoglobulins in a mammal. In particular, the present invention relates to a method for the generation of single chain camelid VHH antibodies in a mammal which undergo the process of class-switching and affinity maturation found within antibody producing B cells. Single chain antibodies generated using the method of the present invention and the uses thereof are also described.
Claims6
A method for the production of a single heavy chain antibody comprising:
- (i) immunizing a transgenic mouse whose genome comprises a heterologous VHH heavy chain locus with an antigen, wherein the heterologous VHH heavy chain locus comprises:
- (a) at least one VHH exon, at least one D exon and at least one J exon, wherein the VHH exon, the D exon and the J exon are capable of recombining to form a VDJ coding sequence, and wherein the VHH exon comprises a naturally occurring camelid VHH coding sequence,
- (b) at least one constant heavy chain region comprising at least one constant heavy chain gene, wherein each of said at least one constant heavy chain gene, when expressed, does not express a functional CH1 domain, and
- (c) a locus control region providing for expression of the VHH heavy chain locus specifically in B cells, wherein said mouse expresses the VHH heavy chain locus in B cells in response to antigen challenge;
- and
- (ii) isolating single heavy chain antibody against said antigen.
3 more elements
Dependencies are inferred from the claim text.