Detergent removal from protein samples prior to mass spectrometry analysis
AssigneePierce Biotechnology, Inc.
InventorsBabu S. Antharavally, A. Krishna Mallia, Ashok Salunkhe
Life of this patent
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Abstract
A method to remove detergents from a biological protein sample that is to be analyzed by mass spectroscopy (MS). The method uses a high performance cyclodextrin polymer resin to remove detergents, which interfere with MS analysis, from a sample containing proteins, peptides, amino acids, etc.
Claims9
A method to prepare a biological sample for mass spectroscopy analysis, the sample containing at least one protein and at least one detergent, the method comprising
- incubating the sample with a cyclodextrin polymer resin polymerized to form a resin bead under conditions sufficient to separate at least 95% to 99% of the detergent from the sample and recover at least 84% to 99% of the protein from the sample, wherein the cyclodextrin polymer resin is (2-hydroxyethyl)-β-cyclodextrin (HEBC).
A method to prepare a biological sample for mass spectroscopy analysis, the sample containing at least one protein and at least one detergent, the method comprising incubating the sample with a (2-hydroxyethyl)-β-cyclodextrin (HEBC) under conditions sufficient to separate at least 95% of the detergent from the sample and recover at least 84% of the protein from the sample.
Dependencies are inferred from the claim text.