System and method of verification of a prepared sample for a flow cytometer
AssigneeAccuri Cytometers, Inc.
InventorsJack T. Ball, Grant C. Howes, Collin A. Rich
Life of this patent
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Abstract
A system and method for a flow cytometer system including a prepared sample fluid with reference beads; an interrogation zone that analyzes the prepared sample fluid; a peristaltic pump system that draws the sample fluid through the interrogation zone; and a processor that monitors a measured volume of sample fluid sampled by the peristaltic pump system and an expected sample volume based on data generated by the analysis of the sample fluid. A system and method is additionally described using an alternative volume sensing fluidic system.
Claims16
A method for verifying preparation of a sample fluid drawn into an interrogation zone of a flow cytometer, comprising:
- receiving the sample fluid prepared with a reference bead concentration;
- drawing a sample volume of the sample fluid with the reference bead concentration into the interrogation zone with a peristaltic pump system;
- analyzing the sample volume of the sample fluid in the interrogation zone to determine a parameter indicative of reference bead count;
- determining an expected value of the sample volume based upon the parameter and the reference bead concentration;
- determining a measured value of the sample volume of the sample fluid used to determine the expected value;
- and
- performing an error correction action in response to a comparison between the measured value of the sample volume and the expected value of the sample volume.
A method for verifying preparation of a sample fluid drawn into an interrogation zone of a flow cytometer, comprising:
- drawing a sample volume of the sample fluid, prepared with a reference bead concentration, into the interrogation zone with a peristaltic pump system;
- analyzing the sample volume of the sample fluid in the interrogation zone to determine a parameter indicative of reference bead count;
- determining an expected vale of the sample volume based on the parameter and the reference bead concentration;
- determining a measured value of the sample volume of the sample fluid used to determine the expected value;
- and
- providing a user notification in response to a comparison between the measured value and the expected value of the sample volume.
Dependencies are inferred from the claim text.