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US 8,507,278 B2Granted
Live attenuated catfish vaccine and method of making
AssigneeMississippi State University
InventorsMark L. Lawrence, Attila Karsi
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Abstract
A high throughput bioluminescence mutant screening procedure is disclosed. This procedure utilizes robotics, and bacterial luciferase to allow real-time monitoring of mutant viability. The procedure was used to develop a live attenuated vaccine for a catfish against E. ictaluri, which is further claimed herein. Additionally, genes from other bacterial species are disclosed which may also be used to create vaccines.
Claims10
A method for identifying bacterial mutants susceptible to host defenses for use in attenuated vaccine development:
- (a) introducing random transposon mutations into bacterial strains expressing bacterial luciferase operon;
- (b) introducing host defense mechanisms to an assay containing said bacterial strains, wherein said host defense mechanisms are selected from the group consisting of serum and neutrophils;
- (c) screening for said mutations by measuring reduction in bioluminescence;
- and
- (d) screening mutants for vaccine efficacy in vivo.
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Dependencies are inferred from the claim text.