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US 8,507,266 B2Granted
Apparatus and method for tissue engineering
AssigneeCase Western Reserve University
InventorsJean F. Welter, Luis A. Solchaga, Jim A. Berilla, Kitsie Penick
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Abstract
A bioreactor system includes a housing and a hydrostatic loading module. The housing includes a chamber with an inlet port and an outlet port. The inlet and outlet ports allowing the chamber to be continuously perfused with a culture medium while chamber is hydrostatically loaded.
Claims44
A bioreactor comprising:
- a housing defining a first chamber that contains a first liquid medium, the housing including an inlet port and an outlet port for fluid flow of the liquid medium through the first chamber, the liquid medium including at least one of a growth or culture medium for growing or culturing cells in the first chamber;
- at least one gas permeable membrane defining at least a portion of the housing, the membrane allowing gas flow through the housing into the first chamber;
- and
- a hydrostatic loading module that transmits hydrostatic pressure through the membrane to the first liquid medium contained in the first chamber.
1 more element
A bioreactor comprising:
- a housing defining a first chamber, a second chamber, and a first gas permeable membrane separating the first chamber and the second chamber and allowing gas flow between the first chamber and the second chamber, the first chamber containing a first liquid medium and including an inlet port and an outlet port for fluid flow of the first liquid medium through the chamber, the first liquid medium being used to culture or grow cells or tissue in the first chamber, the second chamber containing a second liquid medium and including an inlet and outlet for fluid flow of the second liquid medium through the second chamber;
- the hydrostatic pressure of the second liquid medium being transmitted through the first gas permeable membrane to affect the hydrostatic pressure of the first liquid medium contained in the first chamber.
A bioreactor comprising:
- a housing defining a first chamber that contains a first liquid medium and a plurality of cells, the housing including an inlet port and an outlet port for fluid flow of the liquid medium through the first chamber, the liquid medium including at least one of a growth or culture medium for growing or culturing the plurality of cells in the first chamber;
- at least one gas permeable membrane defining at least a portion of the housing, the membrane allowing gas flow through the housing into the first chamber;
- and
- a hydrostatic loading module for transmitting hydrostatic pressure through the membrane to the first liquid medium and the plurality of cells contained in the first chamber.
1 more element
A method of preparing chondrogenic tissue construct, the method comprising:
- isolating a plurality of mesenenchymal stem cells from bone marrow;
- expanding the mesenchymal stem cells in a culture medium;
- seeding the expanded mesenchymal stem cells onto a construct;
- growing the seeded construct in a chondrogenic medium;
- and
- hydrostatically loading the seeded construct while the seeded construct is grown in the chondrogenic medium.
3 more elements
Dependencies are inferred from the claim text.