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US 8,507,265 B2Granted
High-throughput cell transfection device and methods of using thereof
AssigneeRational Biotechnology, Inc.
InventorYong Huang
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AssigneeRational Biotechnology, Inc.
InventorYong Huang
Estimating the term…
Transfecting biology cells with nucleic acid molecules (DNA, siRNA) is an essential prerequisite in elucidating how genes function in complex cellular context and how their activities could be modulated for therapeutic intervention. Traditionally studies are carried out on a low throughput gene-by-gene scale, which has created a huge bottleneck in functional genomic study and drug discovery. Development of high-throughput cell transfection technology will permit functional analysis of massive number of genes and how their activities could be modulated by chemical or biological entities inside cells. This invention describes design, construction of device and apparatus for high throughput effective cell transfection. Procedures and protocols for using the device and apparatus are also described in the application. Novel methods of using the device in cell-based assays are also disclosed.
A culture device comprising a first plate joined to a second plate, and a porous membrane fixed between the first and second plates, the first plate having a plurality of openings aligned with a plurality of openings in the second plate that together define wells having a first chamber and a second chamber separated by the porous membrane, wherein (1) the first chamber is adapted to receive and confine a sample of cell culture;
Dependencies are inferred from the claim text.