Back to results
US 8,507,239 B2Granted
Restriction endonucleases and their applications
AssigneeFermentas UAB
InventorsArvydas Lubys, Jolanta Vitkute, Judita Lubiene, Arvydas Janulaitis
Life of this patent
Estimating the term…
Abstract
Provided is a methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease recognizes in a strand of the duplex a 2 to 6 nucleotide recognition sequence comprising a 5-methylcytosine, and cleaves each strand of the duplex at a fixed position outside the recognition sequence.
Claims40
A methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease comprises SEQ ID NO:
- 1 or a variant thereof at least 90% identical to SEQ ID NO:
- 1.
A methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease comprises SEQ ID NO:
- 1, and recognizes in a strand of the duplex a recognition sequence comprising a 5-methylcytosine, and cleaves each strand of the duplex at a fixed position outside the recognition sequence.
An isolated polypeptide comprising SEQ ID NO:
- 1 or a variant thereof at least 90% identical to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.