Isoprene production using the DXP and MVA pathway
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsGopal K. Chotani, Joseph C. McAuliffe, Michael C. Miller, Rachel E. Muir, Dmitrii V. Vaviline, Walter Weyler
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Abstract
The invention provides for methods for producing isoprene from cultured cells using various components of the DXP pathway and MVA pathway, or components associated with the DXP pathway and MVA pathway, iron-sulfur cluster-interacting redox polypeptides, and isoprene synthase. The invention also provides compositions that include these cultured cells.
Claims16
An isolated host cell capable of producing isoprene comprising (i) a heterologous nucleic acid encoding an iron-sulfur cluster-interacting redox polypeptide or a duplicate copy of an endogenous nucleic acid encoding an iron-sulfur cluster-interacting redox polypeptide, and (ii) a non-mevalonate (DXP) pathway polypeptide, a mevalonate (MVA) pathway polypeptide, and an isoprene synthase polypeptide, wherein the cells are capable of producing isoprene, and wherein at least 35% of the isoprene is generated from DXP pathway flux contribution.
A method of producing isoprene, the method comprising
- (a) culturing isolated cells comprising (i) a heterologous nucleic acid encoding an iron-sulfur cluster-interacting redox polypeptide or a duplicate copy of an endogenous nucleic acid encoding an iron-sulfur cluster-interacting redox polypeptide, and (ii) a non-mevalonate (DXP) pathway polypeptide, a mevalonate (MVA) pathway polypeptide, and an isoprene synthase polypeptide under suitable culture conditions for the production of isoprene, and
- (b) producing isoprene, and wherein at least 35% of the isoprene is generated from DXP pathway flux contribution.
Dependencies are inferred from the claim text.