Vectors and yeast strains for protein production: Ca2+ ATPase overexpression
AssigneeGlycoFi, Inc.
InventorByung-Kwon Choi
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Abstract
Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for producing recombinant glycoproteins that have reduced O-glycosylation.
Claims18
A lower eukaryote host cell that belong to the genus of Pichia comprising a nucleic acid molecule encoding at least one endogenous or exogenous Ca2+ ATPase wherein said nucleic acid in the host cell is ectopic and wherein the host cell further comprises a nucleic acid molecule that encodes a recombinant glycoprotein wherein said glycoprotein is produced with a reduced O-linked glycosylation when said Ca2+ ATPase in the host cell is over-expressed.
A method for producing a recombinant protein having reduced O-glycosylation comprising:
- (a) providing a lower eukaryote host cell that belong to the genus of Pichia comprising a nucleic acid molecule encoding at least one endogenous or exogenous Ca2+ ATPase wherein said nucleic acid in the host cell is ectopic;
- (b) a nucleic acid molecule that encodes a recombinant glycoprotein wherein said glycoprotein is produced with a reduced O-linked glycosylation when said Ca2+ ATPase in the host cell is over-expressed:
- and
- (c) growing the host cell under conditions suitable for producing the recombinant protein.
Dependencies are inferred from the claim text.