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AssigneeAltor BioScience Corporation
InventorsHing C. Wong, Peter Rhode, Bai Liu, Xiaoyun Zhu, Kai-ping Han
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The invention provides soluble fusion protein complexes having at least two soluble fusion proteins. The first fusion protein is a biologically active polypeptide covalently linked to an interleukin-15 (IL-15) polypeptide or a functional fragment thereof. The second fusion protein is a second biologically active polypeptide covalently linked to a soluble interleukin-15 receptor alpha (IL-15Rα) polypeptide or a functional fragment thereof. In the complexes of the invention, one or both of the first and second fusion proteins further includes an immunoglobulin Fc domain or a functional fragment thereof; and the first fusion protein binds to the soluble IL-15Rα domain of the second fusion protein to form a soluble fusion protein complex. The invention further provides methods for making and using the complexes of the invention.
A nucleic acid sequence encoding an IL-15 receptor fusion polypeptide comprising an IL-15 sushi-binding domain fused to an Fc domain (IL-15RαSu/Fc), and a variant interleukin-15 polypeptide comprising an N72D mutation (IL-15N72D).
A method of making an IL-15N72D:IL-15RαSu/Fc fusion protein complex, the method comprising:
A method of making an IL-15N72D:IL-15RαSu/Fc fusion protein complex, the method comprising:
A cell comprising a first polynucleotide encoding a variant interleukin-15 (IL-15) comprising an N72D mutation (IL-15N72D) and a second polynucleotide encoding an IL-15 receptor fusion protein comprising an IL-15 sushi-binding domain fused to an Fc domain (IL-15RαSu/Fc).
An isolated fully occupied IL-15N72D:IL-15RαSu/Fc complex comprising:
Dependencies are inferred from the claim text.