Process for the expression of peptides of interest using GCSF as a fusion partner
AssigneeCadila Healthcare Limited
InventorsSanjeev Kumar Mendiretta, Pankaj R. Patel, Sanjay Bandyopadhyay, Vibhor Saraswat, Arun Kumar Singh
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Abstract
The present invention discloses an improved process for the production of desired recombinant peptides from bacterial cells by using G-CSF as a novel fusion partner for their high level expression in these cells. The invention further provides an expression system comprising the fusion protein wherein the G-CSF is operatively linked to the peptide of interest via an enzymatic or chemical cleavage site which can be used to separate the fusion partner from the said peptide.
Claims27
A process for producing a peptide using Granulocyte Colony Stimulating Factor (GCSF) as a fusion partner wherein the GCSF is fused with a peptide of interest, comprising the steps of:
- a) constructing a GCSF expression vector;
- b) inserting the gene encoding the peptide of interest into the GCSF expression vector;
- c) transforming a host cell with the GCSF expression vector which causes the host cell to express the peptide of interest;
- and
- d) purifying the peptide of interest.
Dependencies are inferred from the claim text.