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US 8,507,218 B2Granted
Detection of degradative enzymes in bodily fluids
AssigneeThe Regents of the University of California
InventorsMichael Heller, Geert W. Schmid-Schoenbein, Roy B. Lefkowitz, Benjamin Sullivan
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Abstract
Provided herein are methods useful in detecting degradative enzymes in bodily fluid samples.
Claims25
A method of detecting a degradative enzyme in a bodily fluid sample, said method comprising the steps of:
- (i) contacting said bodily fluid sample with a negatively charged degradative enzyme substrate or neutral degradative enzyme substrate;
- (ii) allowing said degradative enzyme to react with said negatively charged degradative enzyme substrate or neutral degradative enzyme substrate thereby forming a positively charged degradative enzyme product;
- (iii) electrophoretically separating said positively charged degradative enzyme product from said negatively charged degradative enzyme substrate or neutral degradative enzyme substrate;
- and
- (iv) detecting said separated positively charged degradative enzyme product thereby detecting said degradative enzyme in said bodily fluid sample.
2 more elements
Dependencies are inferred from the claim text.