Aptamers selected against live S. pyogenes cells
AssigneeThe Governors of the University of Alberta
InventorsX. Chris Le, Camille Hamula, Xing-Fang Li
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Abstract
The invention is directed to aptamers, methods and kits comprising same for detecting Streptococcus pyogenes. The method of screening a subject for Streptococcus pyogenes involves obtaining a body sample from the subject; contacting the sample or a bacterial culture of the sample with an aptamer or a panel of aptamers specific to S. pyogenes; and detecting the presence or absence of S. pyogenes in the sample or the bacterial culture, wherein binding of the aptamer is indicative of the presence of S. pyogenes.
Claims17
A method of screening a subject for Streptococcus pyogenes comprising the steps of:
- a) obtaining a body sample from the subject;
- b) contacting the sample or a bacterial culture of the sample with an aptamer or a panel of aptamers specific to S. pyogenes, wherein the aptamer or the panel of aptamers comprises the nucleic acid sequence as set forth in any one of SEQ ID NOS:
- 1-32, or a nucleic acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity therewith over the entire length of said any one of SEQ ID NOS:
- 1-32;
- and
- c) detecting the presence or absence of S. pyogenes in the sample or the bacterial culture, wherein binding of the aptamer or the panel of aptamers is indicative of the presence of S. pyogenes.
A method of screening a subject for Group B Streptococcus comprising the steps of:
- a) obtaining a body sample from the subject;
- b) contacting the sample or a bacterial culture of the sample with an aptamer comprising the nucleic acid sequence as set forth in SEQ ID NO:
- 6 or SEQ ID NO:
- 21;
- c) detecting the presence or absence of Group B Streptococcus in the sample or the bacterial culture, wherein binding of the aptamer is indicative of the presence of Group B Streptococcus.
An isolated nucleic acid having the nucleic acid sequence as set forth in any one of SEQ ID NOS:
- 1-32, or having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity therewith over the entire length of said any one of SEQ ID NOS:
- 1-32.
A kit for detecting the presence of S. pyogenes comprising an aptamer or a panel of aptamers wherein the aptamer or the panel of aptamers comprises the nucleic acid sequence as set forth in any one of SEQ ID NOS:
- 1-32, or a nucleic acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity therewith over the entire length of said any one of SEQ ID NOS:
- 1-32, reagents for detecting the binding of the aptamer or panel of aptamers to S. pyogenes, and one or more supports.
Dependencies are inferred from the claim text.