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US 8,507,201 B2Granted
Detection of Bordetella
AssigneesMayo Foundation for Medical Education and Research, Roche Molecular Systems, Inc.
InventorsFranklin R Cockerill, III, Robin Patel, Lynne M Sloan, Sabine Lohmann, Ulrike Salat
Life of this patent
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Abstract
The invention provides methods to detect Bordetella pertussis and/or Bordetella parapertussis in a biological sample. Primers and probes for the differential detection of B. pertussis and B. parapertussis are provided by the invention. Articles of manufacture containing such primers and probes for detecting B. pertussis and/or B. parapertussis are further provided by the invention.
Claims6
An article of manufacture, comprising:
- a pair of IS481 primers, wherein said pair of IS481 primers comprise a first IS481 primer and a second IS481 primer, wherein said first IS481 primer comprises the sequence 5′-CCA GTT CCT CAA GGA CGC-3′ (SEQ ID NO:1), and wherein said second IS481 primer comprises the sequence 5′-GAG TTC TGG TAG GTG TGA GCG TA-3′ (SEQ ID NO:2);
- a pair of IS481 probes, wherein said pair of IS481 probes comprises a first IS481 probe and a second IS481 probe, wherein said first IS481 probe comprises the sequence 5′-CAC CGC TTT ACC CGA CCT TAC CGC CCA C-3′ (SEQ ID NO:3), and wherein said second IS481 probe comprises the sequence 5′-GAC CAA TGG CAA GGC TCG AAC GCT TCA TC-3′ (SEQ ID NO:11);
- and
- a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.
1 more element
An article of manufacture, comprising:
- a pair of IS481 primers and a pair of IS481 probes,
- wherein said pair of IS481 primers comprise a first IS481 primer and a second IS481 primer, wherein said first IS481 primer comprises the sequence 5′-CCA GTT CCT CAA GGA CGC-3′ (SEQ ID NO:1), and wherein said second IS481 primer comprises the sequence 5′-GAG TTC TGG TAG GTG TGA GCG TA-3′ (SEQ ID NO:2);
- wherein said pair of IS481 probes comprises a first IS481 probe and a second IS481 probe, wherein said first IS481 probe comprises the sequence 5′-CAC CGC TTT ACC CGA CCT TAC CGC CCA C-3′ (SEQ ID NO:3), and wherein said second IS481 probe comprises the sequence 5′-GAC CAA TGG CAA GGC TCG AAC GCT TCA TC-3′ (SEQ ID NO:11);
- and
- a donor fluorescent moiety and a corresponding acceptor fluorescent moiety;
- and
- a pair of IS1001 primers and a pair of IS1001 probes,
- wherein said pair of IS1001 primers comprise a first IS1001 primer and a second IS1001 primer, wherein said first IS1001 primer comprises the sequence 5′-GGC GAT ATC AAC GGG TGA-3′ (SEQ ID NO:5), and wherein said second IS1001 primer comprises the sequence 5′-CAG GGC AAA CTC GTC CAT C-3′ (SEQ ID NO:6);
- wherein said pair of IS1001 probes comprise a first IS1001 probe and a second IS1001 probe, wherein said first IS1001 probe comprises the sequence 5′-GGT TGG CAT ACC GTC AAG A-3′ (SEQ ID NO:12), and wherein said second IS1001 probe comprises the sequence 5′-GCT GGA CAA GGC TCG-3′ (SEQ ID NO:13);
- and
- a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.
8 more elements
Dependencies are inferred from the claim text.