Compositions and methods for making and using multifunctional polymerized liposomes
AssigneeUniversity of Houston
InventorsChengzhi Cai, Guoting Qin, Amit Kumar
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Abstract
According to some embodiments, the present invention provides compositions and methods for making and using multifunctional polymerized liposomes finding relevant application in medical sciences, particularly in bioimaging, diagnostics, drug delivery, and drug formulation. The compositions and methods involve lipids that are both polymerizable and have a “clickable” group that provides the ability to functionalize via a click reaction with various functional moieties useful for the above-listed applications.
Claims9
A method for making multifunctional polymerized liposomes comprising the steps of:
- providing a lipid solution comprising clickable lipids of the general formula R1—(CH2)m—≡—≡—((CH2)n—R2, wherein R1 and R2 are organic groups containing at least one clickable group chosen from an ethynyl group and an azido group, and m and n are integers that vary independently from 1-30;
- treating the lipid solution to form clickable liposomes;
- polymerizing the clickable liposomes by cooling and UV light irradiation;
- and clicking the polymerized clickable liposomes with the at least one clickable group chosen from an azido group and an ethynyl group in the presence of a catalyst for the click reaction;
- wherein the catalyst comprises a compound formed in situ by the addition of a water soluble triazole ligand and ascorbic acid to copper sulfate, wherein the water soluble triazole ligand comprises oligo-ethylene glycol.
The method according to 1, further comprising the step of adding additional molecules chosen from peptides, polymers, sterols and substituted oxysterols.
Dependencies are inferred from the claim text.