Humanized monoclonal antibodies that specifically bind and/or neutralize japanese encephalitis virus (JEV) and their use

An isolated humanized monoclonal antibody that specifically binds Japanese encephalitis virus envelope protein with a binding affinity of about 1.0 nM or less, or an antigen binding fragment thereof, wherein the monoclonal antibody comprises a heavy chain and a light chain, and wherein the monoclonal antibody comprises one of:

• (a) a heavy chain comprising amino acids 31-35 of SEQ ID NO:
• 1, amino acids 50-66 of SEQ ID NO:
• 1, and amino acids 96-107 of SEQ ID NO:
• 1 and a light chain comprising amino acids 24-34 of SEQ ID NO:
• 4, amino acids 50-56 of SEQ ID NO:
• 4, and amino acids 89-96 of SEQ ID NO:
• 4;
• (b) a heavy chain comprising amino acids 32-36 of SEQ ID NO:
• 2, amino acids 50-67 of SEQ ID NO:
• 2, and amino acids 100-113 of SEQ ID NO:
• 2 and a light chain comprising amino acids 24-34 of SEQ ID NO:
• 5, amino acids 50-56 of SEQ ID NO:
• 5, and amino acids 89-96 of SEQ ID NO:
• 5;
• or
• (c) a heavy chain comprising amino acids 30-37 of SEQ ID NO:
• 3, amino acids 52-67 of SEQ ID NO:
• 3, and amino acids 100-112 of SEQ ID NO:
• 3 and a light chain comprising amino acids 22-33 of SEQ ID NO:
• 6, amino acids 49-55 of SEQ ID NO:
• 6, and amino acids 88-95 of SEQ ID NO:
• 6.