Methods for expansion and analysis of cultured hematopoietic stem cells
AssigneeWhitehead Institute for Biomedical Research
InventorsChengcheng Zhang, Harvey F. Lodish
Life of this patent
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Abstract
Methods and kits for propagating hematopoietic stem cells are provided. The methods comprise culturing cells in medium comprising one or more angiopoietin-like proteins, under conditions sufficient for expansion of HSCs. Angiopoietin-like proteins include angiopoietin-like protein 2, angiopoietin-like protein 3, angiopoietin-like protein 4, angiopoietin-like protein 5, angiopoietin-like protein 7, and microfibrillar-associated glycoprotein (Mfap4). Methods for identifying hematopoietic stem cells are provided and isolated hematopoietic stem cells are also provided.
Claims19
A method of propagating hematopoietic stem cells (HSCs) in vitro comprising culturing one or more cells comprising an HSC in a serum free culture medium comprising an angiopoietin-like protein, wherein the serum free culture medium further comprises at least one additional growth factor selected from the group consisting of insulin-like growth factor (IGF), fibroblast growth factor (FGF), thrombopoietin (TPO), and stem cell factor (SCF).
A method of propagating hematopoietic stem cells (HSCs) comprising culturing one or more primary cells comprising an HSC for at least five days in a serum free culture medium comprising an angiopoietin-like protein selected from the group consisting of angiopoietin-like protein 2, angiopoietin-like protein 3, angiopoietin-like protein 4, angiopoietin-like protein 5, angiopoietin-like protein 7, and Mfap4, wherein the serum free culture medium further comprises at least one additional factor selected from the group consisting of insulin-like growth factor (IGF), fibroblast growth factor (FGF), thrombopoietin (TPO), and stem cell factor (SCF).
A method of administering hematopoietic stem cells to an individual comprising:
- a) obtaining hematopoietic stem cells from the individual or a donor;
- b) propagating the hematopoietic stem cells in a serum free culture medium comprising an angiopoietin-like protein and at least one additional factor selected from the group consisting of insulin-like growth factor (IGF), fibroblast growth factor (FGF), thrombopoietin (TPO), stem cell factor (SCF);
- and
- c) transplanting the propagated cells into the individual.
Dependencies are inferred from the claim text.