Flash chromatography cartridge

A low pressure liquid chromatographic cartridge kit including, a polypropylene container and a polypropylene cap for attachment to said container by a user of the kit to form a chromatographic cartridge, wherein:

• said container comprises a hollow cylinder with a longitudinal axis and said cap has an inlet at which fluid is introduced for performing low pressure liquid chromatography when said cap is attached at an upstream end of said container, said container having a downstream end with an outlet for the fluid;
• said container includes a chromatographic packing material placed therein before said kit is obtained by the user, said packing material having a surface spaced from said upstream end of said container to form a predetermined volume between said surface and said cap sufficient to hold a sample for chromatographic analysis when said cap is attached to said container;
• said cap includes a depending skirt having a longitudinal threaded portion on an internal surface thereof and said container has a longitudinal threaded portion on an external surface thereof proximate to said upstream end for engaging said threaded portion on said skirt to enable the user to screw said cap into a final position on said container after obtaining said kit and inserting the sample in said volume, said cap single cap flange depending from the interior of said cap, said cap flange extending downstream beyond the most upstream end of said threaded portion on said skirt;
• and
• a seal capable of withstanding pressures up to about 100 psi is provided between said container and said cap when said cap is screwed into said final position by the user, said seal being formed at least between (a) an inner surface of said container proximate to said upstream end thereof, said container including a lip portion surrounding said inner surface of said upstream end and decreasing in thickness toward said upstream end to present an inclined flat inner surface facing toward said longitudinal axis and extending to said upstream end, and (b) said single cap flange, said cap flange being disposed for accepting said container between said depending skirt and said cap flange, with said flat inner surface of said container lip portion contacting said cap flange at a downstream end of said cap flange when said cap is in an initial position as it is threaded onto said container and said cap flange is bent inwardly toward said longitudinal axis as said cap is further screwed onto said container and into said final position.