Hydrolysis-resistant polyacrylamide gels
AssigneeBio-Rad Laboratories, Inc.
InventorsCraig Rowell, Cory Panattoni, Tom R Berkelman, Sean Cater, Shane Petersen, Lee Olech, Xuemei Yang
Life of this patent
Estimating the term…
Abstract
Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
Claims13
A method for separating a mixture of proteins by electrophoresis, said method comprising (i) loading a gel with a sample of said mixture wherein said gel comprises crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel either:
- having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)aminomethane, and having about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane;
- or having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)aminomethane, and being devoid of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane;
- or being devoid of tris(hydroxymethyl)aminomethane, and having about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, and (ii) imposing a voltage across said gel to cause said proteins to migrate through said gel at differential rates until separated into bands within said gel, said gel having been cast and stored for at least three days prior to steps (i) and (ii).
Dependencies are inferred from the claim text.