Methods of treating a biomass for enzymatic hydrolysis
AssigneeGeorgia Tech Research Corporation
InventorsPrabuddha Bansal, Andreas Sebatian Bommarius, Melanie Hall, Jay Hyung Lee
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Abstract
The present invention is a process for treating a feedstock comprising holocellulose. The process comprises mixing the feedstock with a solution comprising cellulose binding domains to form a mixture. The mixture is then subjected to conditions sufficient to reduce the crystallinity of holocellulose. Subsequent enzymatic hydrolysis may show an improved rate and/or fermentable sugar yield as compared to processes which do not employ the process.
Claims24
A pre-treatment process for treating a feedstock comprising holocellulose wherein the process comprises:
- a) mixing the feedstock with a solution comprising cellulose binding domains to form a mixture;
- and
- b) subjecting the mixture to conditions sufficient to reduce the crystallinity of holocellulose prior to hydrolysis.
A pre-treatment composition suitable for reducing the crystallinity of cellulose prior to hydrolysis, said composition comprising cellulose, a suitable concentration of a solution comprising cellulose binding domains, and a suitable buffer selected from the group consisting of lower alkyl acid salts and lower alkyl acid esters.
Dependencies are inferred from the claim text.