Recombinant elastase proteins and methods of manufacturing and use thereof
AssigneeProteon Therapeutics, Inc.
InventorsF. Nicholas Franano, Kimberly S. Bland, Marco D. Wong, Bee C. Ding
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Abstract
The present invention relates to methods for the manufacture, purification, formulation, and use of biologically active recombinant elastase proteins. Described are recombinant methods for producing therapeutically useful elastase proteins, as are pharmaceutical compositions comprising said elastase proteins. Novel recombinant elastase proteins and protein preparations are also disclosed. Methods are described for treating and preventing diseases of biological conduits using pharmaceutical compositions containing the elastase proteins of the invention.
Claims31
A pharmaceutical composition comprising a mature, enzymatically active human type I elastase protein comprising an amino acid sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO:1 wherein the amount of trypsin corresponds to less than 5 ng of trypsin per 1 mg of mature elastase protein and wherein said mature elastase protein has an activity of 20 to 40 U/mg of protein as quantified using a colorimetric N-succinyl-Ala-Ala-Ala-pNitroanilide (SLAP) hydrolysis assay.
Dependencies are inferred from the claim text.