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US 8,497,124 B2Granted
Methods and products for reprogramming cells to a less differentiated state
AssigneeFactor Bioscience Inc.
InventorsMatthew Angel, Christopher Rohde
Life of this patent
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Abstract
The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.
Claims14
A method for reprogramming a cell to a less differentiated state, comprising:
- a. culturing a differentiated cell with a reprogramming medium containing albumin, wherein the albumin is treated with an ion-exchange resin or charcoal;
- b. transfecting the cell with one or more synthetic RNA molecules, wherein the one or more synthetic RNA molecules includes at least one RNA molecule encoding one or more reprogramming factors and wherein the transfecting results in the cell expressing the one or more reprogramming factors;
- and
- c. repeating step (b) at least twice during 5 consecutive days to result in the cell being reprogrammed to a less differentiated state.
1 more element
A method for reprogramming a cell to a less differentiated state, comprising:
- a. culturing a differentiated cell with a reprogramming medium containing albumin, wherein the albumin is treated with sodium octanoate;
- brought to a temperature of at least 40° C.;
- and treated with an ion-exchange resin or charcoal;
- b. transfecting the cell with one or more synthetic RNA molecules, wherein the one or more synthetic RNA molecules includes at least one RNA molecule encoding one or more reprogramming transcription factors and wherein the transfecting results in the cell expressing the one or more synthetic RNA molecules;
- and
- c. repeating step (b) at least twice during 5 consecutive days to result in the cell being reprogrammed to a less differentiated state.
3 more elements
Dependencies are inferred from the claim text.