Automated tissue engineering system

An automated system for cell culture and/or tissue engineering comprising;

• a) at least two bioreactors linked in sequence by fluid communication comprising:
• i) a first bioreactor for cell or tissue input and/or processing;
• ii) a second bioreactor for cell expansion or forming a tissue construct;
• wherein the cells or tissues input and/or processed in the first bioreactor are passed to the second bioreactor where the cells are expanded or formed into a tissue construct;
• said bioreactors facilitating physiological cellular functions by controlling one or more of the following parameters selected from the group consisting of temperature, pH, dissolved gases, optical density, light scattering, and images of cell/tissue proliferation;
• b) one or more fluid reservoirs in fluid communication with said bioreactors,
• c) the bioreactors comprising one or more sensors sensing, relaying and re-sensing parameters related to said physiological cellular functions and/or generation of cell populations and/or tissue constructs, these parameters selected from the group consisting of temperature, pH, dissolved gases, optical density, light scattering, and images of cell/tissue proliferation;
• d) a microprocessor linked to one or more of said sensors, said microprocessor receiving and monitoring relayed parameters from said sensors such that optimal conditions are maintained in said bioreactors to generate a desired cell population and/or tissue construct and further signals are generated by said microprocessor to re-adjust said parameters of said cell culture and/or tissue engineering process responsive to the status of cell proliferation and/or tissue formation.

An automated cell culture and/or tissue engineering system comprising;

• a) one or more bioreactors each of said bioreactors comprising two or more chambers linked in sequence by fluid communication comprising:
• i. a first chamber for cell or tissue input and/or processing;
• ii. a second chamber for cell expansion or forming a tissue construct;
• for facilitating physiological cellular functions and/or generation of one or more cell populations and/or tissue constructs in a sequential and/or concurrent processing manner within and/or between said chambers and/or bioreactors by controlling one or more of the following parameters selected from the group consisting of temperature, pH, dissolved gases, optical density, light scattering, images of cell/tissue proliferation;
• wherein the cell or tissue input and/or processed in the first chamber are passed to the second chamber where the cells are expanded or formed into a tissue construct;
• b) one or more sensors for monitoring parameters related to said physiological cellular functions and/or generation of cell populations and/or tissue constructs in said sequential and/or concurrent processing manner, selected from the group consisting of temperature, pH, dissolved gases, optical density, light scattering, and images of cell/tissue proliferation;
• wherein said sensors relay said parameters to a microprocessor that reads and then generates further signals and re-adjusts parameters such that optimal conditions are maintained in said bioreactors to generate a desired cell population and/or tissue construct.