Multiple mesodermal lineage differentiation potentials for adipose tissue-derived stromal cells and uses thereof
AssigneeArtecel Sciences, Inc.
InventorsJeffrey Martin Gimble, Yuan-Di Chang Halvorsen, William O. Wilkison
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Abstract
The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair.
Claims10
A medium for differentiating adipose tissue derived stromal cells into hematopoietic supporting stromal cells comprising a chemically defined medium having or supplemented with the following components:
- (i) 0% to 20% fetal bovine serum;
- (ii) antibiotic;
- (iii) interleukins;
- (iv) about 5 pg/ml to 1 ng/ml stem cell factor;
- (v) about 5 pg/ml to 1 ng/ml flt-3ligand;
- (vi) about 5 pg/ml to 1 ng/ml macrophage-colony stimulating factor;
- (vii) about 5 pg/ml to 1 ng/ml granulocyte-monocyte colony stimulating factor;
- (viii) about 5 units/ml to 1000 units/ml erythropoietin;
- (ix) about 5 pg/ml to 1 ng/ml thrombopoietin;
- (x) about 5 pg/ml to 1 ng/ml osteoprotegerin ligand;
- (xi) about 1 nM to 100 nM dexamethasone;
- (xii) about 1 nM to 100 nM hydrocortisone;
- (xiii) about 1 nM to 100 nM 1,25 dihydroxy vitamin D3;
- and (xiv) about 10 uM to 100 uM 2-mercaptoethanol and wherein components (ii)-(xiv) are present.
Dependencies are inferred from the claim text.