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AssigneesUniversity of Medicine and Dentistry of New Jersey, Philadelphia Health and Education Corporation
InventorsIra B. Black, Dale L. Woodbury, Darwin J. Prockop, Emily Schwarz
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The present invention relates to methods of inducing differentiation of mammalian bone marrow stromal cells into neuronal cells by contacting marrow stromal cells with a neuronal differentiation-inducing compounds. Neuronal differentiation-inducing compounds of the invention include anti-oxidants such as, but not limited to, beta-mercaptoethanol, dimethylsulfoxide, butylated hydroxyanisole, butylated hydroxytoluene, ascorbic acid, dimethylfumarate, and n-acetylcysteine. Once induced to differentiate into neuronal cells, the cells can be used for cell therapy, gene therapy, or both, for treatment of diseases, disorders, or conditions of the central nervous system.
A method of inducing differentiation of an isolated marrow stromal cell into a neuronal cell, comprising contacting the isolated marrow stromal cell with at least one growth factor selected from the group consisting of platelet-derived growth factor and fibroblast growth factor 2 and in the absence of further growth or differentiating factors, thereby inducing differentiation of the isolated marrow stromal cell into a neuronal cell.
A method of producing an isolated neuronal cell, comprising isolating a marrow stromal cell, contacting the marrow stromal cell with a neuronal differentiation-inducing compound, wherein the neuronal differentiation-inducing compound is selected from the group consisting of platelet-derived growth factor and fibroblast growth factor 2 and in the absence of further growth or differentiating factors, and wherein the compound induces the isolated marrow stromal cell to differentiate into an isolated neuronal cell, thereby producing said isolated neuronal cell.
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