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US 8,486,675 B2Granted
Protein concentrates and isolates, and processes for the production thereof from macroalgae and/or microalgae
AssigneeBioexx Specialty Proteins Ltd.
InventorQingnong Nelson Tang
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Abstract
Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from macroalgae and/or microalgae using low g-force centrifugation.
Claims46
A process for the production of one or more protein concentrates from defatted or milled macroalgae, microalgae or a combination thereof comprising the steps of:
- i) mixing the macroalgae, microalgae or a combination thereof with a first blending solvent to form a mixture;
- ii) optionally treating the mixture with phytase at a temperature and a pH suitable for phytase activity;
- iii) optionally adjusting the pH of the mixture to solubilize proteins in the mixture;
- iv) subjecting the mixture to a g-force sufficient to separate the mixture to form:
- a) a fiber fraction, and
- b) a protein fraction comprising;
- (i) an insoluble protein fraction, and
- (ii) a soluble protein fraction;
- v) separating the fiber fraction from the protein fraction and mixing the fiber fraction with a second blending solvent to form a fiber mixture;
- vi) treating the fiber mixture with a protease at a temperature and a pH suitable for protease activity;
- vii) subjecting the fiber mixture to a g-force sufficient to separate the fiber mixture to
- form:
- a) a second fiber fraction, and
- b) a hydrolyzed protein fraction, comprising
- (i) an insoluble protein fraction comprising partially hydrolyzed and un-hydrolyzed protein, and
- (ii) a soluble hydrolyzed protein fraction;
- optionally adjusting the pH of the protein fraction from step iv(b) to a pH suitable to precipitate proteins;
- viii) separating the precipitated proteins from the protein fraction, said separated precipitated protein comprising a first protein concentrate;
- and
- ix) optionally combining the first protein concentrate and the hydrolyzed protein fraction to form a second protein concentrate.
17 more elements
A process for the production of one or more protein concentrates from defatted or milled macroalgae, microalgae or a combination thereof comprising the steps of:
- i) mixing the toasted oilseed meal with a first blending solvent to form a mixture;
- ii) optionally treating the mixture with phytase at a temperature and a pH suitable for phytase activity;
- iii) optionally adjusting the pH of the mixture to solubilize proteins in the mixture;
- iv) subjecting the mixture to a g-force sufficient to separate the mixture to form:
- a) a fiber fraction, and
- b) a protein fraction comprising
- (i) an insoluble protein fraction, and
- (ii) a soluble protein fraction;
- v) separating the fiber fraction from the protein fraction and mixing the fiber fraction with a second blending solvent to form a fiber mixture;
- vi) treating the fiber mixture with a protease at a temperature and a pH suitable for protease activity;
- vii) subjecting the fiber mixture to a g-force sufficient to separate the fiber mixture to form:
- a) a second fiber fraction, and
- b) a hydrolyzed protein fraction, comprising
- (i) an insoluble protein fraction comprising partially hydrolyzed and unhydrolyzed protein, and
- (ii) a soluble hydrolyzed protein fraction;
- viii) mixing the protein fraction from step iv(b) with a mixing solvent to precipitate proteins;
- ix) separating the precipitated proteins from the protein fraction, said separated precipitated protein comprising a first protein concentrate;
- and
- x) optionally combining the precipitated proteins first protein concentrate and the hydrolyzed protein fraction to form the a second protein concentrate.
16 more elements
A process for the production of a protein isolate or a hydrolyzed protein isolate from defatted or milled macroalgae, microalgae or a combination thereof comprising the steps of:
- i) mixing macroalgae, microalgae or a combination thereof with a blending solvent to form a mixture;
- ii) optionally treating the mixture with phytase at a temperature and a pH suitable for phytase activity;
- iii) optionally adjusting the pH of the mixture to solubilize proteins in the mixture;
- iv) subjecting the mixture to a g-force sufficient to separate the mixture to form:
- a) a fiber fraction, and
- b) a protein fraction comprising
- (i) an insoluble protein fraction, and
- (ii) a soluble protein fraction;
- v) separating the insoluble protein fraction from the soluble protein fraction to recover therefrom an insoluble protein concentrate and a soluble protein extract;
- and
- vi) subjecting the soluble protein extract to membrane filtration to recover therefrom the protein isolate.
8 more elements
Dependencies are inferred from the claim text.