Back to results
US 8,481,026 B1Granted
Bacteria with increased trehalose production and method for using the same in bioremediation
OwnerPeter J. Woodruff, Tamlyn M. Frederickinventors
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
OwnerPeter J. Woodruff, Tamlyn M. Frederickinventors
Estimating the term…
Bacteria with improved survival under toxic and oxidative stress and methods of using the same for bioremediation are described. Many bacteria such as Escherichia coli have been found to naturally reduce toxic pollutants into a less toxic form, such as by reducing Chromium (VI) to Chromium (III). Reducing such toxins generates damaging reactive oxygen species, so it is important to find a defense for these bacteria against the associated oxidative stress. Trehalose is a small biomolecule that has been shown to protect bacteria from various types of stress by accumulating within the cells. The present invention is directed to bacteria genetically modified to overexpress trehalose biosynthesis genes. The bacteria demonstrate improved viability when challenged with toxins and oxidative stress. The present invention provides inexpensive and beneficial bacteria and methods for environmental remediation.
A genetically modified bacteria demonstrating improved survival under oxidative stress caused by toxins, wherein the genetically modified bacteria includes one or more inducible trehalose biosynthesis genes, resulting in increased trehalose production, wherein the inducible trehalose biosynthesis genes are exogenous to the genetically modified bacteria.
A genetically modified bacteria demonstrating improved survival under oxidative stress caused by toxins, wherein the genetically modified bacteria includes one or more inducible trehalose biosynthesis genes, resulting in increased trehalose production, wherein the inducible trehalose biosynthesis genes include inducible trehalose biosynthesis genes that are both endogenous and exogenous to the genetically modified bacteria.
A method of making a genetically modified bacteria demonstrating improved survival under oxidative stress caused by toxins, the method comprising:
A method of making a genetically modified bacteria demonstrating improved survival under oxidative stress caused by toxins, the method comprising:
A method of reducing the concentration or form of a toxin in an environment, the method comprising:
Dependencies are inferred from the claim text.