Identification of aberrant microarray features
AssigneeAgilent Technologies, Inc.
InventorsPaul Kenneth Wolber, Robert Page
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Abstract
Described herein is a method for identifying an aberrant feature on a nucleic acid array. In general terms, the method comprises: a) obtaining a log transformed normalized value indicating the amount of hybridization of a test sample to a first feature on the nucleic acid array; b) calculating a z-score for the first feature using: the log transformed normalized value; and the distribution of reference log transformed normalized values that indicate the amount of hybridization of control samples to the same feature on a plurality of reference arrays; and c) identifying the test feature as aberrant if it has a z-score that is above or below a defined threshold.
Claims17
A method of identifying an aberrant feature on a nucleic acid array, comprising:
- a) obtaining a log transformed normalized value indicating the amount of hybridization of a test sample to a first feature on said nucleic acid array;
- b) calculating, using a computer, a z-score for said first feature using:
- i. said log transformed normalized value;
- and
- ii. the distribution of reference log transformed normalized values that indicate the amount of hybridization of control samples to the corresponding feature on a plurality of reference arrays;
- and
- c) identifying said test feature as aberrant if it has a z-score that is above or below a defined threshold.
Dependencies are inferred from the claim text.