Reproductive ablation constructs
AssigneeArborgen, LLC
InventorsWilliam H. Rottmann, Kim H. Norris-Caneda, Chunsheng Zhang
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Abstract
The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
Claims20
A method for producing a transgenic plant comprising (a) transforming a plant cell with a construct that comprises (i) a functional Pinus radiata male cone 2 (PrMC2) promoter operably linked to (ii) a polynucleotide that comprises a sequence encoding a K27A barnase mutant;
- and (b) culturing the transformed plant cell under conditions that promote growth of a plant, wherein said plant is a transgenic plant that exhibits a phenotype that is different from a plant of the same species that has not been transformed with the construct.
A construct comprising a Pinus radiata male cone 2 (PrMC2) promoter operably linked to a polynucleotide encoding a K27A barnase mutant, wherein expression of the K27A barnase mutant disrupts reproductive development of at least one of a male reproductive structure in a plant which expresses the construct.
Dependencies are inferred from the claim text.