Method of utilizing the pts gene and anti-sense ads to increase patchouli alcohol content in Artemisia annua L

A method of utilizing the pts gene and antisense ads gene to increase patchouli alcohol content in Artemisia annua L., said method comprising

• a) obtaining the complete open reading frame sequence of artemisinin biosynthesis pathway key enzyme ads gene of SEQ ID NO:
• 1, and the complete open reading frame sequence of the patchouli alcohol biosynthesis pathway key enzyme pts gene, set forth in SEQ ID NO:
• 2, linked to the plastid targeted signal peptide of the Arabidopsis tp gene as set forth in SEQ ID NO:
• 3;
• b) constructing plant expression vectors that contain the ads open reading frame in antisense orientation linked to transcriptional regulatory sequences, and the plastid targeted pts gene linked to transcriptional regulatory sequences;
• c) transforming Agrobacterium tumefaciens simultaneously with expression vectors containing the antisense ads and the plastid targeted pts gene to obtain Agrobacterium tumefaciens for transforming Artemisia annua L. plants;
• d) transforming Artemisia annua L. plants with the vector-transformed Agrobacterium tumefaciens obtained in Step c) and analyzing the transformed plants with PCR to obtain transgenic Artemisia annua L. plants;
• and
• e) determining the patchouli alcohol content in the transgenic Artemisia annua L. plants.