Method of utilizing the PTS gene and RNA interference of the ads gene to increase patchouli alcohol content in Artemisia annua L.

A method of utilizing the pts gene and RNA interference of the ads gene to increase patchouli alcohol content in Artemisia annua L., said method comprising:

• a) obtaining a partial sequence of artemisinin biosynthesis pathway key enzyme ads gene having the sequence of SEQ ID NO:
• 1 that is capable of forming a hairpin structure, and the complete open reading frame sequence of the patchouli alcohol biosynthesis pathway key enzyme pts gene having the sequence of SEQ ID NO:
• 2 that is linked to the nucleotide sequence encoding the plastid targeted signal peptide of the Arabidopsis tp gene having the sequence of SEQ ID NO:
• 3;
• b) obtaining a plant expression vector comprising the partial sequence of the ads gene and a plant expression vector comprising the pts open reading frame linked to the nucleotide sequence encoding the plastid targeted signal peptide;
• c) transforming Agrobacterium tumefaciens simultaneously with the plant expression vectors;
• d) transforming Artemisia annua L. plants with the vector-transformed Agrobacterium tumefaciens obtained in step c) and analyzing the transformed plants with PCR to identify transgenic Artemisia annua L. plants;
• and
• e) determining the patchouli alcohol content in the transgenic Artemisia annua L. plants.