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US 8,476,450 B2Granted
Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
AssigneePromega Corporation
InventorsJames J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
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Abstract
A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
Claims2
A compound according to formula VI:
- wherein R1 is —NRN1RN2;
- wherein RN1 is H, C3-8alkyl which may be substituted with hydroxyl, halo, or amino, or —CH2-A1 or —CH2-A2;
- RN2 is C3-8alkyl which may be substituted with hydroxyl, halo, or amino or —CH2-A1 or —CH2-A2;
- or
- A1 is C6-10aryl or C6-10heteroaryl, which may be substituted with hydroxyl, halo, or amino;
- A2 is C6 aryl or C6 heteroaryl, which may be substituted with hydroxyl, halo, or amino;
- and only one of RN1 or RN2 may be —CH2-A1;
- R6 is COR11 wherein R11 is OH;
- R7 is H.
6 more elements
A compound selected from the group consisting of
Dependencies are inferred from the claim text.