Back to results
US 8,476,436 B1Granted
Method for enhancing luminescence intensity of clytin-II
AssigneeJnc Corporation
InventorsSatoshi Inouye, Yuiko Sahara, Junichi Sato
Life of this patent
Estimating the term…
Abstract
A method for enhancing a luminescence activity of clytin-II is provided. A codon-optimized nucleic acid is used for coding the apo-clytin-II protein, and the luminescent activity of the clytin-II is remarkably enhanced when comparing with the conventional use of the wild-type clytin-II.
Claims8
A method for preparing apo-clytin-II protein, the method comprising:
- (i) preparing a recombinant expression vector that allows a codon-optimized nucleic acid for coding an apo-clytin-II protein under a regulatory control of a promoter that functions in an isolated host cell, wherein said codon optimized nucleic acid is SEQ ID NO:3;
- (ii) introducing the recombinant expression vector into the host cell;
- and
- (iii) culturing the host cell, to allow the apo-clytin-II protein to be expressed.
1 more element
A method for enhancing a luminescence intensity of clytin-II, the method comprising:
- combining a codon-optimized nucleic acid for coding an apo-clytin-II protein with a regulatory nucleic acid for expressing the codon-optimized nucleic acid to form a nucleic acid, wherein said codon optimized nucleic acid is SEQ ID NO:3;
- and
- introducing the resulting nucleic acid into an isolated host cell to code and generate the apo-clytin-II protein in which an expression level of the apo-clytin-II protein is increased so that the luminescence intensity of the clytin-II is enhanced.
A method of a codon-optimized nucleic acid for coding an apo-clytin-II protein, for enhancing a luminescence intensity of clytin-II in host cells, comprising:
- operably linking the codon-optimized nucleic acid of SEQ ID NO:
- 3 that codes for an apo-clytin-II protein with a regulatory nucleic acid to form an expression vector;
- introducing the expression vector into the host cells to code and generate the apo-clytin-II protein, wherein an expression level of the apo-clytin-II protein is increased;
- and
- incubating the host cells in a presence of coelenterazine or a derivative of coelenterazine to convert the apo-clytin-II protein into the clytin-II, wherein the luminescence intensity of the clytin-II is enhanced.
2 more elements
Dependencies are inferred from the claim text.