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AssigneeState of Oregon acting by and through the State Board of Higher Eduction on behalf of Oregon State University
InventorsJames C. Carrington, Edwards Allen
Estimating the term…
A method to generate siRNAs in vivo is described, as are constructs and compositions useful in the method. The method does not depend on the use of DNA or synthetic constructs that contain inverted duplications or dual promoters so as to form perfect or largely double -stranded RNA. Rather, the method depends on constructs that yield single-stranded RNA transcripts, and exploits endogenous or in vivo-produced miRNAs or siRNAs to initiate production of siRNAs. The miRNAs or siRNAs guide cleavage of the transcript and set the register for production of siRNAs (usually 21 nucleotides in length) encoded adjacent to the initiation cleavage site within the construct. The method results in specific formation of siRNAs of predictable size and register (phase) relative to the initiation cleavage site. The method can be used to produce specific siRNAs in vivo for inactivation or suppression of one or more target genes or other entities, such as pathogens.
A plant siRNA-triggering or RNAi-triggering nucleic acid cassette, comprising:
A transgenic plant cell that expresses a first RNA for suppressing a target gene and one or more additional RNAs encoding at least one agronomic trait, wherein the plant cell comprises at least one recombinant DNA molecule from which the first and additional RNAs are transcribed, the recombinant DNA molecule comprising:
A method of simultaneously inhibiting expression of a target gene or sequence in a plant cell and providing an agronomic trait to the plant cell, the method comprising:
A method of producing a stacked trait in a transgenic plant, the method comprising:
A transgenic plant that expresses RNA for suppressing a target gene, wherein the plant comprises recombinant DNA from which there is transcribed a RNA comprising:
Dependencies are inferred from the claim text.