Back to results
US 8,476,415 B2Granted
Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
AssigneeIbis Biosciences, Inc.
InventorsSteven A. Hofstadler, Lendell L. Cummins
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.
Claims11
A kit for purification of nucleic acids comprising:
- a resin source plate containing anion exchange resin, wherein said anion exchange resin comprises amine-terminated magnetic beads, and wherein each of said amine-terminated magnetic beads comprises a magnetic bead linked to at least one amine molecule;
- a sample mixing plate;
- a filter plate or a microtube rack comprising a plurality of micro filter tubes;
- one or more resin wash solutions;
- and
- an electrospray ionization-mass spectrometry-compatible elution buffer, wherein said elution buffer does not comprise a metal cation salt.
3 more elements
Dependencies are inferred from the claim text.