Substrates and internal standards for mass spectrometry detection
AssigneePerkinElmer Health Sciences, Inc.
InventorBlas Cerda
Life of this patent
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Abstract
An inventive substrate is provided which includes a substrate compound of formula A—B1—B2—B3: wherein A is a sugar moiety; B1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B2 contains a permanently charged element such as a quaternary ammonium group so as to increase proton affinities and ionization efficiencies for mass spectrometry detection efficiencies analysis; and B3 of various carbon length conferring specificities to targeted enzymes. Also provided is a process to detect lysosomal diseases by contacting a sample with the inventive substrate along with an internal standard which is isotope-labeled analog of the product cleaved by a targeted enzyme upon the substrate.
Claims15
A substrate for mass spectrometric detection of a lysosomal storage disorder having the formula:
- A—(B1—B2—B3) (I)
- where A is D-glucose or D-galactose, B1 is C1 alkyl or a C6 aryl having a N or O substituent extending from the ring, B2 is an amidyl terminating with a quaternary ammonium or carnitinyl, and B3 is an alkenyl alcohol with a carbon length of 12 to 20 or C2-C20 alkyl having a substituent of O present as a carbonyl,
- where B1 is covalently associated with A by a glycosidic bond;
- said B1 is covalently associated with said A and said B2, and
- said B3 is covalently associated with said B2.
A substrate for mass spectrometric detection of the Krabbe disease having formula of A—(B1—B2—B3) wherein group A is β-D-galactose, B1 is C1 alkyl, B2 is an amidyl terminating with a quaternary ammonium, and B3 is an alkenyl alcohol with a carbon length of 12 to 20,
- where A and B1 are associated by a glycosidic bond.
A substrate for mass spectrometric detection of the Gaucher disease having formula of A—(B1—B2—B3) wherein group A is β-D-glucose, B1 is C1 alkyl, B2 is an amidyl terminating with a quaternary ammonium, and B3 is an alkenyl alcohol with a carbon length of 12 to 20,
- where A and B1 are associated by a glycosidic bond.
Dependencies are inferred from the claim text.